Synthesis of Isopropyl Ferulate Using Silica-Immobilized Lipase in an Organic Medium
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Synthesis of Isopropyl Ferulate Using Silica-Immobilized Lipase in an Organic Medium
Immobilization of lipases has proved to be a useful technique for improving an enzyme's activity in organic solvents. In the present study, the performance of a silica-immobilized lipase was evaluated for the synthesis of isopropyl ferulate in DMSO. The biocatalyst was cross-linked onto the matrix with 1% glutaraldehyde. The effects of various parameters, molar ratio of ferulic acid to isopropy...
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Ascorbyl palmitate was synthesized using a Celite-immobilized commercial lipase (Lipolase 100L) in dimethylsulfoxide (DMSO) as an organic solvent system. Lipase immobilized by surface adsorption onto Celite 545 matrix and subsequently exposed to 1 % glutaraldehyde showed 75 % binding of protein. The Celite-bound lipase was optimally active at 75 °C and pH 8.5 under shaking and showed maximum hy...
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ژورنال
عنوان ژورنال: Enzyme Research
سال: 2011
ISSN: 2090-0414
DOI: 10.4061/2011/718949